UNG enzyme

【Product introduction】

Uracil-n-glycosylase (UNG enzyme), also known as uracil DNA glycosylase (UDG enzyme), is a recombinant enzyme expressed and purified by E.coli. The protein has a molecular weight of 25 KD. It can catalyze the release of free uracil from single and double stranded DNA containing uracil, and has no activity on RNA. It is mainly used to prevent the contamination of PCR amplification products. The mechanism of the enzyme is: in the PCR reaction, dUTP is used to replace dTTP, and all T in the amplified product is replaced by U, and the PCR product containing dU base is formed. UNG enzyme can selectively break the glycosidic bond of U base in single and double stranded DNA, degrade the DNA containing U in the reaction system, effectively eliminate the residual pollution of PCR products, and greatly reduce the false caused by the contamination of amplification products in order to ensure the specificity and accuracy of amplification.

Large purchase volume, more favorable price